PEC (PCR Enhancer Cocktails)

PCR enhancer cocktails, PECs
PCR Enhancer Cocktails (PEC)

We developed our PCR Enhancer Cocktails (PEC) to overcome PCR inhibitors and to permit PCR amplification of GC-rich targets. Combined with our inhibitor-resistant Taq mutants, OmniTaq or Omni Klentaq, PEC enables efficient amplification of exogenous and endogenous targets directly from crude samples containing human plasma, serum, and whole blood without DNA purification. These enhancer cocktails enable the Taq mutants to tolerate at least 25% plasma, serum, or whole blood and as high as 80% GC-content templates. PEC improves real-time PCR performance directly in crude samples with both SYBR Green fluorescence and TaqMan assays. PEC also facilitates DNA amplification from crude samples using most commercial Taq polymerases. (However, PEC does not perform well with AmpliTaq Gold).

 

Selecting the Proper PCR Enhancer

Product Names

PEC-1

PEC-1-GC

PEC-2

PEC-2-GC

Cat#
E600 E610 E620 E630

Purified DNA

-

-

Heparin treated blood

-

-

Citrate treated blood

EDTA treated blood

Heparin treated plasma

-

-

Citrate treated plasma

-

-

EDTA treated plasma

-

-

Serum

-

-

GC-rich target

-

-

Non GC-rich target

-

-

 

GC = PEC for high GC-content targets

 

Generally, if you are working with PCR samples containing purified DNA, we recommend PEC-1 or, for high GC-content targets, PEC-1-GC. Otherwise, for direct amplification of samples containing whole blood, plasma, or serum, we recommend either PEC-1 / PEC-1-GC or PEC-2 / PEC-2-GC, depending on the anticoagulant used and GC content of the target. Please see the table above for details to choose the proper enhancer for you.  If you are unsure which PEC product will work best for you, we now offer the PEC Combo, which contains one tube of each of the four PEC products.