RockStart Performance with Full Length Taq Mutants
![RockStart Buffer provides a Hot Start with Full Length Taq Mutant Enzymes](/sites/default/files/media/images/RockStart%20full%20length.jpg)
Duplicate 340 bp targets were amplified from 10 ng human DNA with the full length Taq mutants; OmniTaq, OmniTaq 2, OmniTaq 3 and CesiumTaq, in Rockstart buffer or standard PCR buffer. A hot start competitor with aptamer technology was used as a positive control. PCR reactions were incubated either at 25 degree or 4 degree for 1 hour before PCR cycling. Results: All full length enzymes show hot start performance with Rockstart buffer with predictably better results when kept at a cooler temperature. Weaker bands with CesiumTaq may be due to lower enzyme concentration. Enzyme titration is always recommended for best results.
RockStart Performance with Truncated Klentaq Mutant Enzymes
![RockStart Buffer provides a Hot Start with Truncated Klentaq Mutant Enzymes](/sites/default/files/media/images/RockStart%20truncated.jpg)
Duplicate 340 bp targets were amplified from 10 ng human DNA using Rockstart buffer or standard buffer. In this gel, the truncated mutant enzymes Klentaq1, Omni Klentaq, Omni Klentaq 2 and Cesium Klentaq AC were tested. A hot start competitor with aptamer technology was used as a positive control. PCR reactions were incubated either at 25 degree or 4 degree for 1 hour before PCR cycling. Results: Klentaq1 and Klentaq mutant enzymes show hot start performance with Rockstart buffer with predictably better results when kept at a cooler temperature. Weaker bands with Cesium Klentaq AC may be due to lower enzyme concentration. Enzyme titration is always recommended for best results.