During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up. This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature. But using a hot start product makes the process much easier.
Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases. They provide an automatic hot start for PCR due to suppressed activity at low temperatures.
RockStart buffer is designed to provide a hot-start with any DNA polymerase.
| Product Name | Cat # | Amount | Price | Purchase |
|---|---|---|---|---|
| Hot Start Klentaq1 | HS100 | 100 ul (2,000 X 25 ul rxns up to 1kb) | $265.00 | |
| Hot Start OmniTaq | HS300 | 125 ul (500 x 25 ul rxns) | $280.00 | |
| Hot Start OmniTaq 2 | HS302 | 125 ul (500 X 25 ul rxns) | $350.00 | |
| Hot Start OmniTaq 3 | HS303 | 125 ul (500 X 25 ul rxns) | $350.00 | |
| Hot Start Omni Klentaq | HS340 | 125 ul (500 X 25 ul rxns) | $280.00 | |
| Hot Start Omni Klentaq 2 | HS340 | 125 ul (500 X 25 ul rxns) | $350.00 | |
| Hot Start Cesium Klentaq C | HS280 | 100ul (2000 x 25ul rxns) | $220.00 | |
| Hot Start Cesium Klentaq AC | HS240 | 100ul (2000 x 25ul rxns) | $220.00 | |
| Hot Start CesiumTaq | HS200 | 100 ul (2,000 X 25 ul rxns) | $220.00 | |
| Hot Start Enzyme Combo | HS500 | variable | $325.00 | |
| RockStart Buffer System | Rock100 | 100 rxns | $60.00 |