During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up. This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature. But using a hot start product makes the process much easier.
Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases. They provide an automatic hot start for PCR due to suppressed activity at low temperatures.
RockStart buffer is designed to provide a hot-start with any DNA polymerase.
|Product Name||Cat #||Amount||Price||Purchase|
|Cesium Klentaq C||280||100 ul (2,000 X 25 ul rxns)||$190.00|
|Cesium Klentaq C LA||290||100 ul (2,000 X 25 ul rxns)||$190.00|
|Cesium Klentaq AC||240||100 ul (2,000 X 25 ul rxns)||$190.00|
|Cesium Klentaq AC LA||250||100 ul (2,000 X 25 ul rxns)||$190.00|
|CesiumTaq||200||100 ul (2,000 X 25 ul rxns)||$190.00|
|CesiumTaq LA||210||100 ul (2,000 X 25 ul rxns)||$190.00|
|Enzyme Combo LA||510||variable||$280.00|