During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up.  This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature.  But using a hot start product makes the process much easier. 

Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases.  They provide an automatic hot start for PCR due to suppressed activity at low temperatures.

RockStart buffer is designed to provide a hot-start with any DNA polymerase.  

Table of Matching Products
Product Name Cat # Amount Price Purchase
RockStart Rock100 100 rxns $50.00
Cesium Klentaq C 280 100 ul (2,000 X 25 ul rxns) $190.00
Cesium Klentaq C LA 290 100 ul (2,000 X 25 ul rxns) $190.00
Cesium Klentaq AC 240 100 ul (2,000 X 25 ul rxns) $190.00
Cesium Klentaq AC LA 250 100 ul (2,000 X 25 ul rxns) $190.00
CesiumTaq 200 100 ul (2,000 X 25 ul rxns) $190.00
CesiumTaq LA 210 100 ul (2,000 X 25 ul rxns) $190.00
Enzyme Combo 500 variable $280.00
Enzyme Combo LA 510 variable $280.00