During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up. This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature. But using a hot start product makes the process much easier.
Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases. They provide an automatic hot start for PCR due to suppressed activity at low temperatures.
RockStart buffer is designed to provide a hot-start with any DNA polymerase.
| Product Name | Cat # | Amount | Price | Purchase |
|---|---|---|---|---|
| RockStart | Rock100 | 100 rxns | $50.00 | |
| Cesium Klentaq C | 280 | 100 ul (2,000 X 25 ul rxns) | $190.00 | |
| Cesium Klentaq C LA | 290 | 100 ul (2,000 X 25 ul rxns) | $190.00 | |
| Cesium Klentaq AC | 240 | 100 ul (2,000 X 25 ul rxns) | $190.00 | |
| Cesium Klentaq AC LA | 250 | 100 ul (2,000 X 25 ul rxns) | $190.00 | |
| CesiumTaq | 200 | 100 ul (2,000 X 25 ul rxns) | $190.00 | |
| CesiumTaq LA | 210 | 100 ul (2,000 X 25 ul rxns) | $190.00 | |
| Enzyme Combo | 500 | variable | $280.00 | |
| Enzyme Combo LA | 510 | variable | $280.00 |