During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up. This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature. But using a hot start product makes the process much easier.
Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases. They provide an automatic hot start for PCR due to suppressed activity at low temperatures.
Our RockStart buffer can be used with any enzyme to provide an automatic hot start.