During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up.  This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature.  But using a hot start product makes the process much easier. 

Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases.  They provide an automatic hot start for PCR due to suppressed activity at low temperatures.

RockStart buffer is designed to provide a hot-start with any DNA polymerase.  

Table of Matching Products
Product Name Cat # Amount Price Purchase
Hot Start Klentaq1 HS100 100 ul (2,000 X 25 ul rxns up to 1kb) $265.00
Hot Start OmniTaq HS300 125 ul (500 x 25 ul rxns) $280.00
Hot Start OmniTaq 2 HS302 125 ul (500 X 25 ul rxns) $350.00
Hot Start OmniTaq 3 HS303 125 ul (500 X 25 ul rxns) $350.00
Hot Start Omni Klentaq HS340 125 ul (500 X 25 ul rxns) $280.00
Hot Start Omni Klentaq 2 HS340 125 ul (500 X 25 ul rxns) $350.00
Hot Start Cesium Klentaq C HS280 100ul (2000 x 25ul rxns) $220.00
Hot Start Cesium Klentaq AC HS240 100ul (2000 x 25ul rxns) $220.00
Hot Start CesiumTaq HS200 100 ul (2,000 X 25 ul rxns) $220.00
Hot Start Enzyme Combo HS500 variable $325.00
RockStart Buffer System Rock100 100 rxns $60.00