Our 5x ready-to-use PCR kit contains Cesium Klentaq AC, a double cold-sensitive mutant of Klentaq polymerase. Due to its suppressed activity at low temperatures this enzyme is designed for hot-start PCR performance. This kit can be used for conventional as well as real-time PCR. Kits include enzyme and a separate master mix of buffer, and dNTPs. For real-time applications you may need to add a fluorescent dye as an alternative to probes.