Our 5x ready-to-use PCR kit contains Cesium Klentaq C LA, a cold-sensitive mutant of Klentaq1 DNA polymerase. Due to its suppressed activity at low temperatures this enzyme is designed for hot-start PCR performance. It also features the Long-Accurate feature that allows amplification of longer products with higher fidelity and accuracy. This kit can be used for conventional, as well as real-time PCR. For real-time applications you may need to add a fluorescent dye as an alternative to probes. LA enzymes are not recommended for use with dUTP.
Kits include enzyme and a separate master mix of buffer, and dNTPs.