Our 5x ready-to-use PCR kit contains CesiumTaq, a double cold-sensitive mutant of Taq polymerase. Due to its suppressed activity at low temperatures this enzyme is designed for hot-start PCR performance. This kit can be used for conventional, as well as real-time PCR. Kits include enzyme and a separate master mix of buffer, and dNTPs. For real time applications you may need to add a fluorescent dye as an alternative to probes.