Our 5x ready-to-use PCR/RT-PCR kit contains OmniTaq2, a mutant of Taq DNA polymerase that provides strand-displacement and reverse transcriptase activity. It can be used as the sole enzyme in RT-PCR and RT-LAMP assays. In addition, this enzyme provides 2-3x faster PCR and some inhibition-resistance. This kit can be used for conventional as well as real-time PCR. For real-time reactions you may need to add a fluorescent dye as an alternative to probes. This kit is not recommended for RT-LAMP, as the buffer and dNTP concentration are incorrect. Please contact us to discuss kit options for RT-LAMP.
Kits include enzyme and a separate master mix of buffer, and dNTPs.
A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement, Frontiers in Bioengineering and Biotechnology (2021)