Hot Start Cesium Klentaq AC is made with aptamer-based technology, enabling room temperature reaction set-up.
Cesium Klentaq AC is a double cold-sensitive mutant of Klentaq1 (5'-exonuclease deficient Taq polymerase with improved fidelity and thermostability). Due to its suppressed activity at low temperatures this enzyme is designed for hot start PCR performance. Addition of the aptamer further enhances its hot start performance.
The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification.