Hot Start Klentaq LA

Hot Start Klentaq LA is made with aptamer-based technology, enabling room temperature reaction set-up.   

Klentaq1 is a 5′-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability. The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification.   The Long-Accurate feature allows for amplification of longer products with higher fidelity and accuracy.  LA enzymes are not recommended for use with dUTP.

10x buffer composition is: 500 mM Tris-Cl pH 9.2, 160 mM ammonium sulfate, 0.5% Brij 58, and 35 mM magnesium chloride. We also offer (upon request) 10x buffer at pH 7.9 for better fidelity.