Hot Start OmniTaq is made with aptamer-based technology, enabling room temperature reaction set-up.
OmniTaq DNA Polymerase is a triple mutant of Taq polymerase that makes the enzyme resistant to the inhibitory effects of blood, soil and more. It remains functional in up to 20-25% whole blood, especially in the presence of our enhancer products, or in some concentrations of crude soil extract or inhibitory food matrices where other commercial enzymes fail. OmniTaq is extremely sensitive and able to pick up trace amounts of DNA in the sample reaction. OmniTaq managed to amplify successfully a single-copy human gene target from 5 pg input DNA. Another special feature of OmniTaq is its fast running ability. A 600bp bacterial target was amplified with 5 seconds extension time and 35 cycles starting off with 1ng of DNA template.
The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification.