Hot Start OmniTaq 2 is a mixture of OmniTaq 2 and a reversibly binding ssDNA aptamer.
OmniTaq 2 DNA polymerase is a mutant of Taq polymerase that provides 2-3x faster PCR and some inhibition-resistance.
The aptamer forms a hairpin and binds to the active site of the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The aptamer is released at the first melt step, allowing full enzyme activity.
Please note that Hot Start OmniTaq 2 is not yet optimized for use in RT-PCR or RT-LAMP.
We highly recommend pairing this product with one of our PCR Enhancer Cocktails.
The Hot Start version of OmniTaq 2 provides improved amplification for this tough target
See more data on our Performance Data page.