The PCR Enhancer Cocktail Combo contains 1.25 ml (100 x 25 ul rxns) of each of our five PCR Enhancer Cocktails. This will allow you to try all five of the enhancer formulations to see which one is right for you. Please refer to our PCR Enhancer Cocktail information page for more information and for help selecting the right PCR Enhancer Cocktail for your application.
Our PCR enhancer cocktails: In an attempt to overcome PCR inhibition, enhance PCR amplification, and simplify the PCR protocol, we developed PCR enhancing cocktails, PEC-1 and PEC-2. Our cocktails, in combination with our inhibitor-resistant Taq mutants enable efficient amplification of exogenous, endogenous, and, in their high GC versions, as high as 80% GC content templates. In the presence of these enhancer cocktails, the mutant Taq enzymes can tolerate higher percentages of plasma, serum, or whole blood.
The PEC-P enhancer is specially formulated for polyphenolic, humic acid, and bile/feces samples.
These enhancer cocktails also improved the performance of the novel Taq mutants in real-time PCR amplification using crude samples, both in SYBR Green fluorescence detection and TaqMan assays. The novel enhancer mixes facilitate DNA amplification from crude samples with most commercial Taq DNA polymerases. PEC-1 and PEC-2 however do NOT perform well with AmpliTaq Gold.