Our 5x ready-to-use PCR kit contains Hot Start OmniTaq 3, made with aptamer-based technology, enabling room temperature reaction set-up.
OmniTaq 3 is a mutant of Taq polymerase that makes the enzyme resistant to the inhibitory effects of blood, soil, and more. It remains functional in up to 40% whole blood, especially in the presence of our enhancer products, or in some concentrations of crude soil extract or inhibitory food matrices where other commercial enzymes fail.
This kit can be used for conventional as well as real-time PCR. For real time reactions you may need to add a fluorescent dye as an alternative to probes.
Kits include enzyme and a separate master mix of buffer, and dNTPs.