Hot Start CesiumTaq is made with aptamer-based technology, enabling room temperature reaction set-up.
CesiumTaq is a double cold-sensitive mutant of Taq DNA polymerase. Due to its suppressed activity at low temperatures this enzyme is designed for hot-start PCR performance. Addition of the aptamer further enhances its hot start capabilities.
The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. 10x buffer composition is: 500 mM Tris-Cl pH 8.3, 160 mM ammonium sulfate, 0.25% Brij 58, and 25 mM magnesium chloride.