Hot Start CesiumTaq LA is made with aptamer-based technology, enabling room temperature reaction set-up.

CesiumTaq is a double cold-sensitive mutant of Taq DNA polymerase. Due to its suppressed activity at low temperatures this enzyme is designed for hot-start PCR performance.  Addition of the aptamer further enhances its hot start performance.The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The Long-Accurate feature allows for amplification of longer products with higher fidelity and accuracy.  LA enzymes are not recommended for use with dUTP.  

10x buffer composition is: 500 mM Tris-Cl pH 8.3, 160 mM ammonium sulfate, 0.25% Brij 58, and 25 mM magnesium chloride. 

Catalog Number
100 ul (2,000 X 25 ul rxns)