… compete for the signal which is to be detected, and/or 2) some chemicals in the samples can interfere with (inhibit) … provide the best possible functionality. Regarding problem 2) it is well-established that blood contains inhibitors to …
… were 25 ul and 0.125 ul of each enzyme was used. PCR reactions were performed in duplicate. A competitor Hot Start Taq was also included. The PCR mix were stored at 4°C or incubated at 25°C for 1 hour prior to PCR. *A555 is one of our in-progress enzymes. Results : …
… N-terminal deletion (removing the 5'-[flap]exonuclease of Taq DNA polymerase). PCR tests show that Klentaq1 has … thermostability (about 2 degrees) relative to wild-type Taq, and it gives higher yields of amplicon. We speculate …
… Each tube of 1.5 ml of 10x Klentaq1 Reaction Buffer pH 9.2 is enough for 600 25 ul PCR reactions. 10x buffer … 0.5% Brij 58, and 35 mM magnesium chloride. Our 10x Klentaq1 Reaction Buffer pH 7.9 also available for better … Datasheet Klentaq1 Reaction Buffer 9.2 …
… improved fidelity (about 25%) and thermostability (about 2 degrees) relative to wild-type Taq, and it gives higher … (equivalent to 200 ul standard enzyme. Volume may be up to 2.5x higher) …
… 10x Klentaq1 Reaction Buffer pH 7.9 is enough for 600 25ul PCR reactions. 10x buffer composition is 500 mM Tris-Cl, 160 … specifically for Klentaq1. It is distinct from the Klentaq Mutant Reaction Buffer that is designed for CesiumKlentaq, Omni Klentaq, and other Klentaq mutants. …
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