… It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes … polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The aptamer is released at the first melt step, allowing full enzyme activity. RockStart buffer is designed to provide a …
… recommended for GC-rich targets amplified from purfied DNA, blood treated with heparin, citrate, or EDTA, or plasma … In many cases amplification may be performed without DNA purification . They are highly recommended for use with … PECs are compatible with most commercially available DNA polymerases, but they are not recommended for use with …
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